A genomic day in the life of a clinical microbiology laboratory.

VNTR9 and VNTR10, two newly-found variable-number tandem repeat loci useful in MLVA genotyping of Bordetella pertussis

Background & Aims: Bordetella pertussis, the causative agent of whooping cough, continues to infect human hosts even in those populations where infants and children are routinely vaccinated. Causes of pertussis epidemiology are not fully identified unless strains of the pathogen are characterized by molecular means. Golbally, Multi Locus Variable Number of Tandem Repeats analysis (MLVA) has pro...

Genomic Linkage Analysis of Iranian Clinical Isolates of Dermatophytes Fungi Using the RAPD-PCR

Dermatophytes are a group of keratinophilic fungi capable of invading keratinized tissues (skin, hair and nails). They cause dermatophytosis (commonly known as tinea or Ring worm) in human and animals. In this report, DNA similarities and genomic linkage of 40 dermatophytes strains was obtained from different universities, were studied by random amplified polymorphic DNA (RAPD–PCR) using 11 ra...

Introducing New Laboratory-Developed Molecular Methods in the Clinical Microbiology Laboratories

Chemical synthesis of Zinc Oxide (ZnO) nanoparticles and their antibacterial activity against a clinical isolate Staphylococcus aureus

In this study, Zinc Oxide nanoparticles (ZnONPs) were synthesized by chemical method and their antibacterial efficiency against a clinical isolate of Staphylococcus aureus was studied. The Zinc Oxide nanoparticles have shown a commendable inhibition effect on the growth of most notorious bacterial pathogen S.aureus. The nanoparticles size and shapes were characterized by trans...

Evaluation of anaerobic pathogens in periodontitis patients and its relationship with TGF-1β genomic polymorphism by Tetra Arms-PCR method

Background and Aims: Periodontitis is a common and inflammatory infectious disease that causes damage to the tissues supporting the tooth and consequent tooth loss. Periodontal disease is a multimicrobial and multifactorial disease and important anaerobic bacteria are involved in periodontal infection. TGF-1β is one of the growth factors and anti-inflammatory cytokines that play a crucial role ...